Pseudomonas putida bacteria: the biotech industry's obsession with the organism's fundamental carbon and cellular biochemistry
1. Ahmed Nabawy, Darrang College, Tezpur, Student, India
Pseudomonas putida, a rod-shaped microorganism that belongs to the genus Negative bacteria, is very adaptable. Their metabolism has developed to cope with environmental and chemical stress, allowing them to live and even flourish under extreme conditions. This quality allows it to survive even in harsh environments. Research into the bacterium's potential industrial uses has so intensified in recent years. Other important factors are the use of waste materials and inexpensive natural biomass substrate to create value-added compounds. This progress is being driven by systems bioinformatics, which combines systems genetic analysis techniques with new fission reactors and renewable biomaterials. Systems genomics is one factor fueling this development.
pseudomonads bacterium bacteria microbial microbes bacterial organism biofilm
The
product line of P. putida, like those of many industrialized microorganisms,
has undergone significant evolution in recent times. The high genetic openness
and inherent resistance it has seem to be advantageous for coping with the
poisonous and harsh conditions involved with industrial bioprocessing and the
de-novo creation of often synthetic chemicals. It's important to remember that
this versatile bacterium is employed in a wide range of industrial
biotechnology processes. P. putida is a key player in the industrial
manufacture of chiral compounds because to its diverse enzyme repertory.
Potential for P. putida's use to expand in industrial biotechnology is high
because of the exciting new products that are becoming accessible thanks to the
proliferation of efficient P. putida cellular factory. P. putida pipes that use
renewable hydrocarbons or industrial effluent for sustainability bio-production
are pictured, and such an integration might be an exciting future development.
P. putida's vast intrinsic route repertory allows it to breakdown and
metabolise a wide variety of compounds, including sophisticated aromatic
hydrocarbons.
P.
putida is capable of utilising such substrates, therefore it might potentially
convert aromatic chemicals present in lignocellulose from fast pyrolysis into
value-added products like cis-cis muconate, a great starting material for the
production of adipic acid. In addition to its potential utility in the sugar
industry, P. putida shows promise as a tool for creating novel compounds or
materials. P. putida's high resistance to environmental hazards, along with its
amenability to genetic engineering, makes it a promising candidate for use as a
platform for producing otherwise unattainable synthetic chemicals. Experimental
and analytical systems level approaches will be necessary to decipher the
intricacy of the central and peripheral biosynthetic processes in P. putida and
regulate them. Through methods like fine-tuning expression and managing gene
regulation or integrating complex allogeneic pathways, tissue engineering will
allow for a new level of ductility in redesigning metabolism for greater
bio-production. P. putida has a bright future thanks to the combination of
rational strained engineering and revolutionary concepts or genetic
engineering, which will lead to strains with even better tolerance to specific
operating conditions and more widespread use in industry.
1. Yuan H, Liu H, Du J, Liu K, Wang T, Liu L (2020) Biocatalytic production of 2,5-furandicarboxylic acid: recent advances and future perspectives. Appl Microbiol Biotechnol 104(2):527–543. https://doi. org/10.1007/s00253-019-10272-9
The author confirms sole responsibility for the following: study conception and design, data collection, analysis and interpretation of results, and manuscript preparation.
The authors did not receive any specific grants from funding agencies in the public, commercial, or non-profit sectors for the research, authorship, and/or publication of this article.
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All authors declare that they have no conflicts of interest.
I thank the following individuals for their expertise and assistance in all aspects of our study and for their help in writing the manuscript. I am also grateful for the insightful comments given by anonymous peer reviewers. Everyone's generosity and expertise have improved this study in myriad ways and saved me from many errors.
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