Plant tissue culture is a collection of techniques used to
maintain or grow plant cells, tissues or organs under sterile conditions on a
nutrient culture medium of known composition. It is widely used to produce
clones of a plant in a method known as micropropagation. Different techniques
in plant tissue culture may offer certain advantages over traditional methods
of propagation, including:
- The production of exact copies of plants that produce
particularly good flowers, fruits, or have other desirable traits.
- To quickly produce mature plants.
- The production of multiples of plants in the absence of
seeds or necessary pollinators to produce seeds.
- The regeneration of whole plants from plant cells that
have been genetically modified.
- The production of plants in sterile containers that
allows them to be moved with greatly reduced chances of transmitting diseases,
pests, and pathogens.
- The production of plants from seeds that otherwise have
very low chances of germinating and growing, i.e. orchids and Nepenthes.
- To clean particular plants of viral and other infections
and to quickly multiply these plants as 'cleaned stock' for horticulture and
Plant tissue culture relies on the fact that many plant
cells have the ability to regenerate a whole plant (totipotency). Single cells,
plant cells without cell walls (protoplasts), pieces of leaves, stems or roots
can often be used to generate a new plant on culture media given the required
nutrients and plant hormones.
- Plant tissue laboratory and aseptic techniques
- Preparation and composition of nutrient media
- Mass micropropagation of healthy plants
- Callus and cell culture
- Somaclonal variation
- Experiments in plant tissue culture
- Meristem culture
- Generative organ cultures
- Vegetative organ cultures
- Production of biochemical via cell culture
- Somatic embryogenesis
- Protoplast culture
- Gene gun transformation
- Validation of transgene integration
- Plant tissue cultures in horticulture