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<article xlink="http://www.w3.org/1999/xlink" mml="http://www.w3.org/1998/Math/MathML" xsi="http://www.w3.org/2001/XMLSchema-instance" ali="http://www.niso.org/schemas/ali/1.0/" noNamespaceSchemaLocation="http://jats.nlm.nih.gov/publishing/1.1/xsd/JATS-journalpublishing1-mathml3.xsd" article-type="research-article" dtd-version="1.1" lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">isrdo-SRJEEP</journal-id><journal-id journal-id-type="pmc">isrdo-SRJEEP</journal-id><journal-id journal-id-type="nlm-ta">isrdo-SRJEEP</journal-id><journal-title-group><journal-title>Scientific Research Journal of Environment, Earth and Physical Science</journal-title><abbrev-journal-title abbrev-type="publisher" pub-type="epub">SRJEEP</abbrev-journal-title></journal-title-group><issn>2584-0614</issn><publisher><publisher-name>ISRDO</publisher-name><publisher-loc>Gujarat,India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">M-10267</article-id><article-id pub-id-type="doi"/><article-categories><subj-group subj-group-type="categories"><subject>Environment Science and technology</subject></subj-group></article-categories><title-group><article-title>Literature Review: Genomic Profiling for Monitoring Antimicrobial Resistance in Environmental Wastewater</article-title></title-group><contrib-group content-type="authors"><contrib id="434" contrib-type="author" corresp="yes"><name><given-names>Prakash Chheatry</given-names></name><xref ref-type="aff" rid="aff-1">1</xref><aff id="aff-1"><label>0</label><institution>Nagaland University</institution><country>India</country></aff></contrib><contrib id="435" contrib-type="author" corresp="yes"><name><given-names>Manimala P</given-names></name><xref ref-type="aff" rid="aff-2">2</xref><aff id="aff-2"><label>1</label><institution>Nagaland University</institution><country>India</country></aff></contrib></contrib-group><contrib-group content-type="editors"><contrib contrib-type="editor"/></contrib-group><pub-date pub-type="epub" data-type="pub" iso-8601-date="2025-07-16"><day>16</day><month>07</month><year iso-8601-date="2">2025</year></pub-date><volume>3</volume><elocation-id>V3-I1-2025</elocation-id><history><date date-type="received" iso-8601-date="2025-04-29"><day>29</day><month>04</month><year iso-8601-date="2025">2025</year></date><date date-type="revised" iso-8601-date="2025-06-12"><day>12</day><month>06</month><year iso-8601-date="2025"/></date><date date-type="accepted" iso-8601-date="2025-06-12"><day>12</day><month>06</month><year iso-8601-date="2025"/></date></history><permissions><copyright-statement>&#xA9;2025 Prakash Chheatry Year Corresponding Author</copyright-statement><copyright-year>2025</copyright-year><copyright-holder>Prakash Chheatry</copyright-holder><license href="https://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (ISRDO) and either DOI or URL of the article must be cited.<ext-link ext-link-type="uri" href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link></license-p></license></permissions><self-uri href="https://isrdo.org/journal/SRJEEP/currentissue/literature-review-genomic-profiling-for-monitoring-antimicrobial-resistance-in-environmental-wastewater"/><abstract><p>Both human and environmental health are seriously jeopardised by the rise of antimicrobial resistance (AMR).&nbsp; The monitoring of antimicrobial resistance (AMR) in populations has become increasingly dependent on wastewater-based surveillance.&nbsp; One promising comprehensive high-throughput approach to identifying and measuring resistance genes is metagenomics, which entails sequencing and analysing genetic material from environmental samples.&nbsp; Nevertheless, its efficacy is constrained by methodological obstacles such inconsistent bioinformatics, low-abundance gene detection, and environmental heterogeneity.&nbsp; Findings from important research on AMR monitoring using metagenomics in wastewater are synthesised in this review.&nbsp; It emphasises new developments such as coupled qPCR-metagenomic methods for higher sensitivity and specificity, better bioinformatics pipelines, and synthetic DNA standards.&nbsp; Machine learning for pattern identification and worldwide surveillance networks for coordinated monitoring are two options proposed in the study to fill up the gaps in standardisation and data interpretation.&nbsp; In order to make metagenomic AMR monitoring in wastewater settings more accurate and scalable, it is essential to establish uniform procedures and to increase the worldwide surveillance infrastructure.</p></abstract><kwd-group kwd-group-type="author"><kwd>Antimicrobial resistance</kwd><kwd> wastewater surveillance</kwd><kwd> metagenomics</kwd><kwd> quantitative metagenomics</kwd><kwd> qPCR</kwd><kwd> bioinformatics</kwd><kwd> low-abundance gene detection</kwd></kwd-group><funding-group><funding-statement>No grants from public, commercial, or non-profit funding agencies supported the research, authorship, or publication of this article.</funding-statement></funding-group></article-meta></front><back><sec sec-type="data-availability"><title>Data Availability</title><p>Not applicable&nbsp;&nbsp;&nbsp;&nbsp;</p></sec><sec sec-type="COI-statement"><title>Conflicts of Interest</title><p>There are no conflicts of interest declared by the authors.</p></sec><sec sec-type="author-contributions"><title>Authors&#x2019; Contributions</title><p>The author independently managed the study's conception, design, data acquisition, analysis, and manuscript drafting.</p></sec><sec sec-type="funding-statement"><title>Funding Statement</title><p>No grants from public, commercial, or non-profit funding agencies supported the research, authorship, or publication of this article.</p></sec><sec sec-type="software-information"><title>software-information</title><p>Not applicable</p></sec><ack><title>Acknowledgments</title><p>My gratitude goes to those who assisted in this study and manuscript preparation, and to the anonymous reviewers for their constructive insights.</p></ack><ref-list content-type="authoryear"><ref id="1"><label>1</label><element-citation publication-type="journal"><p>-</p></element-citation></ref></ref-list></back></article>
