TY - M-10062
AU - Khan, Samiya
TI - Impact of Pretreatment Methods on Chromosome Stability in Regenerated Plantlets
T2 - Scientific Research Journal of  Biology and Life Science

PY - 2023
VL - 1
IS - 2
SN - 2584-0606

AB - The root tips of various in vitro-cultivated young plants,
encompassing Anogeissus species, Capparis decidua, and Maytenus emarginata,
underwent treatment with colchicine and exposure to cold temperatures at -4°C
overnight. Following these preliminary treatments, the plant's root tips were
preserved using a combination of glacial sulfuric acid and absolute ethanol in
a 1:3 proportion. After fixation, the root tips were stained with a 0.5%
solution of basic fuchsine in preparation for chromosome analysis, involving
the creation of squash preparations. These dyed root tips were gently pressed
in a 1% acetocarmine solution with zinc chloride as a mordant. Subsequently,
the produced slides were scrutinized under a microscope. The results revealed
that most in vitro-cultivated plantlets exhibited chromosomal stability, while
a minority of the seedlings displayed aneuploidy.

KW - chromosome stability
KW -  regenerated plantlets
KW -  pretreatment methods
KW -  cytological analysis
KW -  genetic integrity
KW -  chromosomal count
DO -