@Article{M-10062,
AUTHOR = {Khan, Samiya},
TITLE = {Impact of Pretreatment Methods on Chromosome Stability in Regenerated Plantlets},
JOURNAL = {Scientific Research Journal of  Biology and Life Science},
VOLUME = {1},
YEAR = {2023},
NUMBER = {2},
ARTICLE-NUMBER = {M-10062},
URL = {https://isrdo.org/journal/SRJBL/currentissue/impact-of-pretreatment-methods-on-chromosome-stability-in-regenerated-plantlets},
ISSN = {2584-0606},
ABSTRACT = {The root tips of various in vitro-cultivated young plants,
encompassing Anogeissus species, Capparis decidua, and Maytenus emarginata,
underwent treatment with colchicine and exposure to cold temperatures at -4°C
overnight. Following these preliminary treatments, the plant's root tips were
preserved using a combination of glacial sulfuric acid and absolute ethanol in
a 1:3 proportion. After fixation, the root tips were stained with a 0.5%
solution of basic fuchsine in preparation for chromosome analysis, involving
the creation of squash preparations. These dyed root tips were gently pressed
in a 1% acetocarmine solution with zinc chloride as a mordant. Subsequently,
the produced slides were scrutinized under a microscope. The results revealed
that most in vitro-cultivated plantlets exhibited chromosomal stability, while
a minority of the seedlings displayed aneuploidy.},
DOI = {}
}