The root tips of various in vitro-cultivated young plants, encompassing Anogeissus species, Capparis decidua, and Maytenus emarginata, underwent treatment with colchicine and exposure to cold temperatures at -4°C overnight. Following these preliminary treatments, the plant's root tips were preserved using a combination of glacial sulfuric acid and absolute ethanol in a 1:3 proportion. After fixation, the root tips were stained with a 0.5% solution of basic fuchsine in preparation for chromosome analysis, involving the creation of squash preparations. These dyed root tips were gently pressed in a 1% acetocarmine solution with zinc chloride as a mordant. Subsequently, the produced slides were scrutinized under a microscope. The results revealed that most in vitro-cultivated plantlets exhibited chromosomal stability, while a minority of the seedlings displayed aneuploidy.
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The author confirms sole responsibility for the following: study conception and design, data collection, analysis and interpretation of results, and manuscript preparation.
The authors did not receive any specific grants from funding agencies in the public, commercial, or non-profit sectors for the research, authorship, and/or publication of this article.
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I thank the following individuals for their expertise and assistance in all aspects of our study and for their help in writing the manuscript. I am also grateful for the insightful comments given by anonymous peer reviewers. Everyone's generosity and expertise have improved this study in myriad ways and saved me from many errors.
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